Carcinogenesis, Teratogenesis & Mutagenesis ›› 2004, Vol. 16 ›› Issue (2): 78-80.doi: 10.3969/j.issn.1004-616x.2004.02.003

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The Inhibition of Suppressor Gene KAI1 and ATRA on the Small Lung Cancer Cell

WANG Ying; XIE Juan; SUN Shi-liang; et al   

  1. The Medical University of Chong Qing, Chongqing 400016,China
  • Received:2003-10-20 Revised:2003-11-25 Online:2004-03-30 Published:2004-03-30
  • Contact: WANG Ying

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Abstract: BACKGROUND ＆ AIM: To investigate the inhibition of suppressor gene KAI1 in the process of all－trans－retinoic acid on the NCI－H446 small lung cancer cell. MATERIAL AND METHODS:To establish NCI－H446 cell line with stably expressing tumor suppressor gene KAI1,the gene KAI1 was transfected into NCI－H446 cell by the Vector ector(PCMV－NEO－XhoI).We obtained clone cells after selected by G418.After that,the cells were treated with ATRA at the dosage of 10－6 mol/L.The proliferation and differentiation of the cells were examined by the methods of the clone formation and cytometry analysis. The CD82 protein expression was detected by cytometry analysis,The change of MYC was tested by immunohistochemical staining and the expression of LN was tested by radioimmunoassay. RESULTS: The CD82 protein expression was up－regulated in the cells treated by ATRA.There were more cells arrested in G1/G0 phase.The expressions of MYC and LN were descended. CONCLUSION:The suppressor gene KAI1 cooperate with ATRA in inhibiting proliferation and promoting differentiation of the Small Cell Lung cell.

Key words: suppressor gene KAI1, all－trans－retinoicacid, small cell lung cancer